Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX.

ABSTRACT: We present a rapidly neutralizable and highly anticoagulant thrombin-binding aptamer with a short toehold sequence, originally discovered by systematic evolution of ligands by exponential enrichment (SELEX) with microbead-assisted capillary electrophoresis (MACE). MACE is a novel CE-partitioning method for SELEX and able to separate aptamers from a library of unbound nucleic acids, where the aptamer and target complexes can be detected reliably and partitioned with high purity even in the first selection cycle. Three selection rounds of MACE-SELEX discovered several TBAs with a nanomolar affinity (K_d = 4.5-8.2 nM) that surpasses previously reported TBAs such as HD1, HD22, and NU172 (K_d = 118, 13, and 12 nM, respectively). One of the obtained aptamers, M08, showed a 10

SUBMITTER: Wakui K 

PROVIDER: S-EPMC6462803 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature