Myricanol rescues dexamethasone-induced muscle dysfunction via a sirtuin 1-dependent mechanism.

ABSTRACT:

Background

Muscle atrophy and weakness are adverse effects of high dose or the sustained usage of glucocorticoids. Loss of mitochondria and degradation of protein are highly correlated with muscle dysfunction. The deacetylase sirtuin 1 (SIRT1) plays a vital role in muscle remodelling. The current study was designed to identify myricanol as a SIRT1 activator, which could protect skeletal muscle against dexamethasone-induced wasting.

Methods

The dexamethasone-induced atrophy in C2C12 myotubes was evaluated by expression of myosin heavy chain, muscle atrophy F-box (atrogin-1), and muscle ring finger 1 (MuRF1), using western blots. The mitochondrial content and oxygen consumption were assessed by MitoTracker staining and extracellular flux analysis, respectively. Muscle dysfunct

SUBMITTER: Shen S 

PROVIDER: S-EPMC6463464 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature