N^6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation.

ABSTRACT: N⁶-Methyladenosine (m⁶A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m⁶A modifications have been extensively studied and shown to play critical roles in many cellular processes, the identity of m⁶A methyltransferases for rRNAs and the function of rRNA m⁶A modifications are unknown. Here we report a new m⁶A methyltransferase, ZCCHC4, which primarily methylates human 28S rRNA and also interacts with a subset of mRNAs. ZCCHC4 knockout eliminates m⁶A4220 modification in 28S rRNA, reduces global translation, and inhibits cell proliferation. We also find that ZCCHC4 protein is overexpressed in hepatocellular carcinoma tumors, and ZCC

SUBMITTER: Ma H 

PROVIDER: S-EPMC6463480 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature