Selective translational usage of TSS and core promoters revealed by translatome sequencing.

ABSTRACT:

Background

In mammals, fine-tuned regulation of gene expression leads to transcription initiation from diverse transcription start sites (TSSs) and multiple core promoters. Although polysome association is a critical step in translation, whether polysome selectively uses TSSs and core promoters and how this could impact translation remains elusive.

Results

In this study, we used CAGE followed by deep sequencing to globally profile the transcript 5' isoforms in the translatome and transcriptome of human HEK293 cells at single-nucleotide resolution. By comparing the two profiles, we identified the 5' isoforms preferentially used in translatome and revealed a widespread selective usage of TSSs (32.0%) and core promoters (48.7%) by polysome. We discovered the transcription initi

SUBMITTER: Li H 

PROVIDER: S-EPMC6463679 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature