Human cytomegalovirus-specific T-cell receptor engineered for high affinity and soluble expression using mammalian cell display.

ABSTRACT: T-cell receptors (TCR) have considerable potential as therapeutics and antibody-like reagents to monitor disease progression and vaccine efficacy. Whereas antibodies recognize only secreted and surface-bound proteins, TCRs recognize otherwise inaccessible disease-associated intracellular proteins when they are presented as processed peptides bound to major histocompatibility complexes (pMHC). TCRs have been primarily explored for cancer therapy applications but could also target infectious diseases such as cytomegalovirus (CMV). However, TCRs are more difficult to express and engineer than antibodies, and advanced methods are needed to enable their widespread use. Here, we engineered the human CMV-specific TCR RA14 for high-affinity and robust soluble expression. To achieve this, we adapte

SUBMITTER: Wagner EK 

PROVIDER: S-EPMC6463697 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature