Zinc transporter 10 (ZnT10)-dependent extrusion of cellular Mn²⁺ is driven by an active Ca²⁺-coupled exchange.

ABSTRACT: Manganese (Mn²⁺) is extruded from the cell by the zinc transporter 10 (ZnT10). Loss of ZnT10 expression caused by autosomal mutations in the ZnT10 gene leads to hypermanganesemia in multiple organs. Here, combining fluorescent monitoring of cation influx in HEK293-T cells expressing human ZnT10 with molecular modeling of ZnT10 cation selectivity, we show that ZnT10 is exploiting the transmembrane Ca²⁺ inward gradient for active cellular exchange of Mn²⁺ In analyzing ZnT10 activity we used the ability of Fura-2 to spectrally distinguish between Mn²⁺ and Ca²⁺ fluxes. We found that (a) application of Mn²⁺-containing Ca²⁺-free solution to ZnT10-expressing cells triggers an influx of Mn²⁺, (

SUBMITTER: Levy M 

PROVIDER: S-EPMC6463715 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature