MiR-19 promotes the proliferation of clear cell renal cell carcinoma by targeting the FRK-PTEN axis.

ABSTRACT:

Background

The non-receptor tyrosine kinase Fyn-related kinase (FRK) has been reported to affect cell proliferation in several cancer types. However, its effect on the proliferation of clear cell renal cell carcinoma (ccRCC) remains largely unknown.

Purpose

The objective of this study was to investigate the expression pattern and function of FRK in ccRCC. We further determined how FRK interacted with other molecules to regulate ccRCC proliferation.

Patients and methods

The expression of FRK in ccRCC samples and paired normal renal tissues from 30 patients were analyzed by immunoblotting, immunohistochemistry and quantitative PCR. Then the role of FRK in ccRCC proliferation was analyzed by Cell Counting Kit-8, colony formation assay and EdU incorporation assay. In addi

SUBMITTER: Jing ZF 

PROVIDER: S-EPMC6463782 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature