A network of human functional gene interactions from knockout fitness screens in cancer cells.

ABSTRACT: Genetic interactions mediate the emergence of phenotype from genotype. The systematic survey of genetic interactions in yeast showed that genes operating in the same biological process have highly correlated genetic interaction profiles, and this observation has been exploited to infer gene function in model organisms. Such assays of digenic perturbations in human cells are also highly informative, but are not scalable, even with CRISPR-mediated methods. As an alternative, we developed an indirect method of deriving functional interactions. We show that genes having correlated knockout fitness profiles across diverse, non-isogenic cell lines are analogous to genes having correlated genetic interaction profiles across isogenic query strains and similarly imply shared biological function. We

SUBMITTER: Kim E 

PROVIDER: S-EPMC6464042 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature