Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens.

ABSTRACT: Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40°C, compared to physiological (37°C) or pathophysiological temperatures (42°C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased thei

SUBMITTER: Stan RC 

PROVIDER: S-EPMC6464238 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature