Single cell genomics yields a wide diversity of small planktonic protists across major ocean ecosystems.

ABSTRACT: Marine planktonic protists are critical components of ocean ecosystems and are highly diverse. Molecular sequencing methods are being used to describe this diversity and reveal new associations and metabolisms that are important to how these ecosystems function. We describe here the use of the single cell genomics approach to sample and interrogate the diversity of the smaller (pico- and nano-sized) protists from a range of oceanic samples. We created over 900 single amplified genomes (SAGs) from 8 Tara Ocean samples across the Indian Ocean and the Mediterranean Sea. We show that flow cytometric sorting of single cells effectively distinguishes plastidic and aplastidic cell types that agree with our understanding of protist phylogeny. Yields of genomic DNA with PCR-identifiable 18S rRNA ge

SUBMITTER: Sieracki ME 

PROVIDER: S-EPMC6465268 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature