Directed in Vitro Evolution of Therapeutic Bacteriophages: The Appelmans Protocol.

ABSTRACT: The 'Appelmans protocol' is used by Eastern European researchers to generate therapeutic phages with novel lytic host ranges. Phage cocktails are iteratively grown on a suite of mostly refractory bacterial isolates until the evolved cocktail can lyse the phage-resistant strains. To study this process, we developed a modified protocol using a cocktail of three Pseudomonas phages and a suite of eight phage-resistant (including a common laboratory strain) and two phage-sensitive Pseudomona aeruginosa strains. After 30 rounds of selection, phages were isolated from the evolved cocktail with greatly increased host range. Control experiments with individual phages showed little host-range expansion, and genomic analysis of one of the broad-host-range output phages showed its recomb

SUBMITTER: Burrowes BH 

PROVIDER: S-EPMC6466182 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature