L-Type Voltage-Gated Ca²⁺ Channels Regulate Synaptic-Activity-Triggered Recycling Endosome Fusion in Neuronal Dendrites.

ABSTRACT: The repertoire and abundance of proteins displayed on the surface of neuronal dendrites are tuned by regulated fusion of recycling endosomes (REs) with the dendritic plasma membrane. While this process is critical for neuronal function and plasticity, how synaptic activity drives RE fusion remains unexplored. We demonstrate a multistep fusion mechanism that requires Ca²⁺ from distinct sources. NMDA receptor Ca²⁺ initiates RE fusion with the plasma membrane, while L-type voltage-gated Ca²⁺ channels (L-VGCCs) regulate whether fused REs collapse into the membrane or reform without transferring their cargo to the cell surface. Accordingly, NMDA receptor activation triggered AMPA-type glutamate receptor trafficking to the dendritic surface in an L-VGCC-dependent

SUBMITTER: Hiester BG 

PROVIDER: S-EPMC6466620 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature