Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector.

ABSTRACT:

Background

A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in advanced cellular therapy. Current strategies for producing RBCs have shortcomings in the limited self-renewal capacity of progenitor cells, or difficulties in effectively enucleating erythroid cell lines. We explored a new method to produce RBCs by inducibly expressing c-Myc in primary erythroid progenitor cells and evaluated the proliferative and maturation potential of these modified cells.

Results

Primary erythroid progenitor cells were genetically modified with an inducible gene transfer vector expressing a single transcription factor, c-Myc, and all th

SUBMITTER: Mayers S 

PROVIDER: S-EPMC6466758 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature