Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts.

ABSTRACT:

Background

Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery.

Methods

Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-β1, and HIF1α were determined by RT-PCR and western blotting respectively.

Results

After Cygb overexpression, hTFs displayed no significant cha

SUBMITTER: Wei H 

PROVIDER: S-EPMC6466771 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature