Hepatic DNA methylation and expression profiles under prenatal restricted diet in three generations of female rat fetuses.

ABSTRACT: The nutritional factors acting during early life can affect the development of the organism. It has been hypothesized that such programmed traits can be inherited by later generations. In this work, we present for the first time the effect of food deprivation in pregnant dams and its consequences for the transcription and DNA methylation profiles in the offspring of the next three generations. We used a 50% reduction in dietary intake during pregnancy in the rat and determined whether this altered the hepatic DNA methylation and transcription levels in female fetuses over three generations. Targeted next-generation sequencing (tNGS) was used in the first generation for 1748 genes associated with six selected biological processes. The selected cytosines were then studied by pyrosequencing i

SUBMITTER: Nowacka-Woszuk J 

PROVIDER: S-EPMC6467388 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature