Gene isolation, heterologous expression, purification and functional confirmation of sesamin synthase from Sesamum indicum L.

ABSTRACT: Members of Cytochromes P450 super family of enzymes catalyse important biochemical reactions in plants. Some of these reactions are so important that they contribute to enormous chemical diversity seen in plants. Many unique secondary metabolites formed by mediation of these enzymes play key role in plant defence and often contribute to maintenance of human health. In oilseed crop Sesamum indicum, the reaction leading to the formation of clinically important sesamin is catalyzed by a unique methylene-di-oxy bridge forming Cytochrome P450 enzyme sesamin synthase. It is encoded by the gene CYP81Q1. In order to elucidate the structure - function relationship of this enzyme and to apply biotechnological tools for enhancing the production of sesamin in the crop, it was intended to clone and exp

SUBMITTER: Chandra K 

PROVIDER: S-EPMC6468150 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature