Human H4 tail stimulates HIV-1 integration through binding to the carboxy-terminal domain of integrase.

ABSTRACT: The integration of the retroviral genome into the chromatin of the infected cell is catalysed by the integrase (IN)•viral DNA complex (intasome). This process requires functional association between the integration complex and the nucleosomes. Direct intasome/histone contacts have been reported to modulate the interaction between the integration complex and the target DNA (tDNA). Both prototype foamy virus (PFV) and HIV-1 integrases can directly bind histone amino-terminal tails. We have further investigated this final association by studying the effect of isolated histone tails on HIV-1 integration. We show here that the binding of HIV-1 IN to a peptide derived from the H4 tail strongly stimulates integration catalysis in vitro. This stimulation was not observed with peptide tails from ot

SUBMITTER: Mauro E 

PROVIDER: S-EPMC6468294 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature