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An estimate of the total number of true human miRNAs.

ABSTRACT: While the number of human miRNA candidates continuously increases, only a few of them are completely characterized and experimentally validated. Toward determining the total number of true miRNAs, we employed a combined in silico high- and experimental low-throughput validation strategy. We collected 28 866 human small RNA sequencing data sets containing 363.7 billion sequencing reads and excluded falsely annotated and low quality data. Our high-throughput analysis identified 65% of 24 127 mature miRNA candidates as likely false-positives. Using northern blotting, we experimentally validated miRBase entries and novel miRNA candidates. By exogenous overexpression of 108 precursors that encode 205 mature miRNAs, we confirmed 68.5% of the miRBase entries with the confirmation rate going up to 94.4% for the high-confidence entries and 18.3% of the novel miRNA candidates. Analyzing endogenous miRNAs, we verified the expression of 8 miRNAs in 12 different human cell lines. In total, we extrapolated 2300 true human mature miRNAs, 1115 of which are currently annotated in miRBase V22. The experimentally validated miRNAs will contribute to revising targetomes hypothesized by utilizing falsely annotated miRNAs.

SUBMITTER: Alles J

PROVIDER: S-EPMC6468295 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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An estimate of the total number of true human miRNAs.

Alles Julia J Fehlmann Tobias T Fischer Ulrike U Backes Christina C Galata Valentina V Minet Marie M Hart Martin M Abu-Halima Masood M Grässer Friedrich A FA Lenhof Hans-Peter HP Keller Andreas A Meese Eckart E

Nucleic acids research 20190401 7

While the number of human miRNA candidates continuously increases, only a few of them are completely characterized and experimentally validated. Toward determining the total number of true miRNAs, we employed a combined in silico high- and experimental low-throughput validation strategy. We collected 28 866 human small RNA sequencing data sets containing 363.7 billion sequencing reads and excluded falsely annotated and low quality data. Our high-throughput analysis identified 65% of 24 127 matur ...[more]

PMID: 30820533

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Project description:The volume of securely encrypted data transmission increases continuously in modern society with all things connected. Towards this end, true random numbers generated from physical sources are highly required for guaranteeing security of encryption and decryption schemes for exchanging sensitive information. However, majority of true random number generators (TRNGs) are mechanically rigid, and thus cannot be compatibly integrated with some specific flexible platforms. Herein, we present a flexible and stretchable bionic TRNG inspired by the uniqueness and randomness of biological architectures. The flexible TRNG film is molded from the surface microstructures of natural plants (e.g., ginkgo leaf) via a simple, low-cost, and environmentally friendly manufacturing process. In our proof-of-principle experiment, the TRNG exhibits a fast generation speed of up to 1.04 Gbit/s, in which random numbers are fully extracted from laser speckle patterns with a high extraction rate of 72%. Significantly, the resulting random bit streams successfully pass all randomness test suites including NIST, TestU01, and DIEHARDER. Even after 10,000 times cyclic stretching or bending tests, or during temperature shock (-25-80 °C), the bionic TRNG still reveals robust mechanical reliability and thermal stability. Such a flexible TRNG shows a promising potential in information security of emerging flexible networked electronics.Electronic supplementary materialSupplementary material (light path diagram of transmitted laser speckle, pseudo random pattern, statistical distribution of bionic microstructures, haze of the bionic TRNG film, multi-layer circular laser intensity pattern, percentage of bit 0/1 for different hashed images, Pearson correlation coefficient between 100 different speckle images, the whole process of randomness extraction, SEM images of the flexible TRNG film after 10,000 times stretching and bending, continuous work stability of the TRNG at low or high temperature, light path diagram of reflective laser speckle, and detailed randomness test results of NIST, TestU01, and DIEHARDER) is available in the online version of this article at 10.1007/s12274-022-4109-9.

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An estimate of the total number of true human miRNAs.

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An estimate of the total number of true human miRNAs.

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