Targeted Mass Spectrometry Analysis of Clostridium perfringens Toxins.

ABSTRACT: Targeted proteomics recently proved to be a technique for the detection and absolute quantification of proteins not easily accessible to classical bottom-up approaches. Due to this, it has been considered as a high fidelity tool to detect potential warfare agents in wide spread kinds of biological and environmental matrices. Clostridium perfringens toxins are considered to be potential biological weapons, especially the epsilon toxin which belongs to a group of the most powerful bacterial toxins. Here, the development of a target mass spectrometry method for the detection of C. perfringens protein toxins (alpha, beta, beta2, epsilon, iota) is described. A high-resolution mass spectrometer with a quadrupole-Orbitrap system operating in target acquisition mode (parallel reactio

SUBMITTER: Duracova M 

PROVIDER: S-EPMC6468457 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature