Role of Calcium Signaling in GA101-Induced Cell Death in Malignant Human B Cells.

ABSTRACT: GA101/obinutuzumab is a novel type II anti-CD20 monoclonal antibody (mAb), which is more effective than rituximab (RTX) in preclinical and clinical studies when used in combination with chemotherapy. Ca²⁺ signaling was shown to play a role in RTX-induced cell death. This report concerns the effect of GA101 on Ca²⁺ signaling and its involvement in the direct cell death induced by GA101. We reveal that GA101 triggered an intracellular Ca²⁺ increase by mobilizing intracellular Ca²⁺ stores and activating Orai1-dependent Ca²⁺ influx in non-Hodgkin lymphoma cell lines and primary B-Cell Chronic Lymphocytic Leukemia (B-CLL) cells. According to the cell type, Ca²⁺ was mobilized from two distinct intracellular compartments. In Raji,

SUBMITTER: Latour S 

PROVIDER: S-EPMC6468563 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature