Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions.

ABSTRACT: Enzymatic catalysis is of great importance to the chemical industry. However, we are still scratching the surface of the potential of biocatalysis due to the limited operating range of enzymes in harsh environments or their low recyclability. The role of Metal-Organic Frameworks (MOFs) as active supports to help overcome these limitations, mainly by immobilization and stabilization of enzymes, is rapidly expanding. Here we make use of mild heating and a non-polar medium during incubation to induce the translocation of a small enzyme like protease in the mesoporous MOF MIL-101(Al)-NH₂. Our proteolytic tests demonstrate that protease@MIL-101(Al)-NH₂ displays higher activity than the free enzyme under all the conditions explored and, more importantly, its usability can b

SUBMITTER: Navarro-Sanchez J 

PROVIDER: S-EPMC6469195 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature