Novel CSF biomarkers in genetic frontotemporal dementia identified by proteomics.

ABSTRACT:

Objective

To identify novel CSF biomarkers in GRN-associated frontotemporal dementia (FTD) by proteomics using mass spectrometry (MS).

Methods

Unbiased MS was applied to CSF samples from 19 presymptomatic and 9 symptomatic GRN mutation carriers and 24 noncarriers. Protein abundances were compared between these groups. Proteins were then selected for validation if identified by ≥4 peptides and if fold change was ≤0.5 or ≥2.0. Validation and absolute quantification by parallel reaction monitoring (PRM), a high-resolution targeted MS method, was performed on an international cohort (n = 210) of presymptomatic and symptomatic GRN, C9orf72 and MAPT mutation carriers.

Results

Unbiased MS revealed 20 differentially abundant proteins between

SUBMITTER: van der Ende EL 

PROVIDER: S-EPMC6469343 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature