Identification of pathogenic genes of pterygium based on the Gene Expression Omnibus database.

ABSTRACT:

Aim

To identify the pathogenic genes in pterygium.

Methods

We obtained mRNA expression profiles from the Gene Expression Omnibus database (GEO) to identify differentially expressed genes (DEGs) between pterygium tissues and normal conjunctiva tissues. The Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction (PPI) network and transcription factors (TFs)-target gene regulatory network was performed to understand the function of DEGs. The expression of selected DEGs were validated by the quantitative real-time polymerase chain reaction (qRT-PCR).

Results

A total of 557 DEGs were identified between pterygium and normal individual. In PPI network, several genes were with high degrees such as FN1, KPNB1, DDB1, NF2 and <

SUBMITTER: Yue XL 

PROVIDER: S-EPMC6469547 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature