Cytogenetic, Genomic, and Functional Characterization of Pituitary Gonadotrope Cell Lines.

ABSTRACT: LβT2 and αT3-1 are important, widely studied cell line models for the pituitary gonadotropes that were generated by targeted tumorigenesis in transgenic mice. LβT2 cells are more mature gonadotrope precursors than αT3-1 cells. Microsatellite authentication patterns, chromosomal characteristics, and their intercellular variation have not been reported. We performed microsatellite and cytogenetic analysis of both cell types at early passage numbers. Short tandem repeat (STR) profiling was consistent with a mixed C57BL/6J × BALB/cJ genetic background, with distinct patterns for each cell type. Spectral karyotyping in αT3-1 cells revealed cell-to-cell variation in chromosome composition and pseudodiploidy. In LβT2 cells, chromosome counting and karyoty

SUBMITTER: Ruf-Zamojski F 

PROVIDER: S-EPMC6469952 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature