Identification and Molecular Characterization of Peroxisome Proliferator-Activated Receptor δ as a Novel Target for Covalent Modification by 15-Deoxy-Δ^12,14-prostaglandin J₂.

ABSTRACT: PPARδ belongs to the peroxisome proliferator-activated receptor (PPAR) family of nuclear receptors. Upon activation by an agonist, PPARδ controls a variety of physiological processes via regulation of its target genes. 15-Deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) is a cyclopentenone prostaglandin that features an electrophilic, α,β-unsaturated ketone (an enone) in the cyclopentenone ring. Many of 15d-PGJ₂'s biological effects result from covalent interaction between C₉ and the thiol group of a catalytic cysteine (Cys) in target proteins. In this study, we investigated whether 15d-PGJ₂ activates PPARδ by forming a covalent adduct. Our data show that 15d-PGJ₂ activates PPARδ's transcriptional activity through formati

SUBMITTER: Reddy AT 

PROVIDER: S-EPMC6470001 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature