Application of triple quadrupole mass spectrometry for the characterization of antibody-drug conjugates.

ABSTRACT: Antibody-drug conjugates (ADCs) are an inherently heterogeneous class of biotherapeutics, the development of which requires extensive characterization throughout. During the earliest phases of preclinical development, when synthetic routes towards the desired conjugate are being assessed, the main interest lies in the determination of the average drug-to-antibody ratio (DAR) of a given batch as well as information about different conjugation species. There has been a trend in mass spectrometry (MS)-based characterization of ADCs towards the use of high-resolving mass spectrometry for many of these analyses. Considering the high cost for such an instrument, the evaluation of cheaper and more accessible alternatives is highly motivated. We have therefore tested the applicability of a quadrup

SUBMITTER: Kallsten M 

PROVIDER: S-EPMC6470114 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature