Development and Application of a Functional Human Esophageal Mucosa Explant Platform to Eosinophilic Esophagitis.

ABSTRACT: There is an increasing prevalence of esophageal diseases but intact human tissue platforms to study esophageal function, disease mechanisms, and the interactions between cell types in situ are lacking. To address this, we utilized full thickness human donor esophagi to create and validate the ex vivo function of mucosa and smooth muscle (n = 25). Explanted tissue was tested for contractile responses to carbachol and histamine. We then treated ex vivo human esophageal mucosa with a cytokine cocktail to closely mimic the Th2 and inflammatory milieu of eosinophilic esophagitis (EoE) and assessed alterations in smooth muscle and extracellular matrix function and stiffening. We found that full thickness human esophagus as well as the individual layers of circular and longitudinal muscularis pro

SUBMITTER: Kurten RC 

PROVIDER: S-EPMC6470157 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature