Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments.

ABSTRACT: Although reversible platelet aggregation observed in response to ADP stimulation in the presence of calcium is a well-known phenomenon, its mechanisms are not entirely clear. To study them, we developed a simple kinetic mass-action-law-based mathematical model to use it in combination with experiments. Light transmission platelet aggregometry (LTA) induced by ADP was performed for platelet-rich plasma or washed platelets using both conventional light transmission and aggregate size monitoring method based on optical density fluctuations. Parameter values of the model were determined by means of parameter estimation techniques implemented in COPASI software. The mathematical model was able to describe reversible platelet aggregation LTA curves without assuming changes in platelet aggregatio

SUBMITTER: Filkova AA 

PROVIDER: S-EPMC6470167 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature