Analysis of the In Vivo Transcriptome of Bordetella pertussis during Infection of Mice.

ABSTRACT: Bordetella pertussis causes the disease whooping cough through coordinated control of virulence factors by the Bordetella virulence gene system. Microarrays and, more recently, RNA sequencing (RNA-seq) have been used to describe in vitro gene expression profiles of B. pertussis and other pathogens. In previous studies, we have analyzed the in vitro gene expression profiles of B. pertussis, and we hypothesize that the infection transcriptome profile in vivo is significantly different from that under laboratory growth conditions. To study the infection transcriptome of B. pertussis, we developed a simple filtration technique for isolation of bacteria from infected lungs. The work flow involves filtering the bacteria out of the lung homo

SUBMITTER: Wong TY 

PROVIDER: S-EPMC6470212 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature