Uncovering the hidden marine sponge microbiome by applying a multi-primer approach.

ABSTRACT: Marine sponges (phylum Porifera) are hosts to microorganisms that make up to 40-60% of the mesohyl volume. The challenge is to characterise this microbial diversity more comprehensively. To accomplish this, a new method was for the first time proposed to obtain sequence coverage of all the variable regions of the 16S rRNA gene to analyze the amplicon-based microbiomes of four representative sponge species belonging to different orders. The five primer sets targeting nine variable regions of the 16S rRNA gene revealed a significant increase in microbiome coverage of 29.5% of phylum level OTUs and 35.5% class level OTUs compared to the community revealed by the commonly used V4 region-specific primer set alone. Among the resulting OTUs, 52.6% and 61.3% were unaffiliated, including candidate

SUBMITTER: Yang Q 

PROVIDER: S-EPMC6470215 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature