Modular Enzymatic Cascade Synthesis of Vitamin B₅ and Its Derivatives.

ABSTRACT: Access to vitamin B₅ [(R)-pantothenic acid] and both diastereoisomers of α-methyl-substituted vitamin B₅ [(R)- and (S)-3-((R)-2,4-dihydroxy-3,3-dimethylbutanamido)-2-methylpropanoic acid] was achieved using a modular three-step biocatalytic cascade involving 3-methylaspartate ammonia lyase (MAL), aspartate-α-decarboxylase (ADC), β-methylaspartate-α-decarboxylase (CrpG) or glutamate decarboxylase (GAD), and pantothenate synthetase (PS) enzymes. Starting from simple non-chiral dicarboxylic acids (either fumaric acid or mesaconic acid), vitamin B₅ and both diastereoisomers of α-methyl-substituted vitamin B₅ , which are valuable precursors for promising antimicrobials against Plasmodium falciparum and multidrug-resistant Staphylococcus aureus, can be

SUBMITTER: Abidin MZ 

PROVIDER: S-EPMC6471175 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature