LSPR Biosensing Approach for the Detection of Microtubule Nucleation.

ABSTRACT: Microtubules are dynamic protein filaments that are involved in a number of cellular processes. Here, we report the development of a novel localized surface plasmon resonance (LSPR) biosensing approach for investigating one aspect of microtubule dynamics that is not well understood, namely, nucleation. Using a modified Mie theory with radially variable refractive index, we construct a theoretical model to describe the optical response of gold nanoparticles when microtubules form around them. The model predicts that the extinction maximum wavelength is sensitive to a change in the local refractive index induced by microtubule nucleation within a few tens of nanometers from the nanoparticle surface, but insensitive to a change in the refractive index outside this region caused by microtubule

SUBMITTER: Hasegawa K 

PROVIDER: S-EPMC6471214 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature