Sequencing and Phylogenetic Analysis of Chloroplast Genes in Freshwater Raphidophytes.

ABSTRACT: The complex evolution of chloroplasts in microalgae has resulted in highly diverse pigment profiles. Freshwater raphidophytes, for example, display a very different pigment composition to marine raphidophytes. To investigate potential differences in the evolutionary origin of chloroplasts in these two groups of raphidophytes, the plastid genomes of the freshwater species Gonyostomumsemen and Vacuolariavirescens were sequenced. To exclusively sequence the organelle genomes, chloroplasts were manually isolated and amplified using single-cell whole-genome-amplification. Assembled and annotated chloroplast genes of the two species were phylogenetically compared to the marine raphidophyte Heterosigmaakashiwo and other evolutionarily more diverse microalgae. These phylogenetic comparisons confir

SUBMITTER: Sassenhagen I 

PROVIDER: S-EPMC6471398 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature