Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

ABSTRACT: Zebrafish are well-suited for in vivo calcium imaging because of the transparency of their larvae and the ability to express calcium probes in various cell subtypes. This model organism has been used extensively to study brain development, neuronal function, and network activity. However, only a few studies have investigated calcium homeostasis and signaling in zebrafish neurons, and little is known about the proteins that are involved in these processes. Using bioinformatics analysis and available databases, the present study identified 491 genes of the zebrafish Calcium Toolkit (CaTK). Using RNA-sequencing, we then evaluated the expression of these genes in the adult zebrafish brain and found 380 hits that belonged to the CaTK. Based on quantitative real-time polymerase chain reaction ar

SUBMITTER: Wasilewska I 

PROVIDER: S-EPMC6471419 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature