H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells.

ABSTRACT:

Background

Porcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine.

Methods

Porcine satellite cells (PSCs) were isolated and in-vitro cultured from newborn piglets. RNA Sequencing (RNA-Seq) and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers-H3K4me3, H3K27me3, and H3K27ac-and RNA polymerase II.

Results

The study identified 917 differentially expressed genes during cell differentiation. The landscape of epigeneti

SUBMITTER: Wang S 

PROVIDER: S-EPMC6471710 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature