Metaproteomic and 16S rRNA Gene Sequencing Analysis of the Infant Fecal Microbiome.

ABSTRACT: A metaproteomic analysis was conducted on the fecal microbiome of eight infants to characterize global protein and pathway expression. Although mass spectrometry-based proteomics is now a routine tool, analysis of the microbiome presents specific technical challenges, including the complexity and dynamic range of member taxa, the need for well-annotated metagenomic databases, and high inter-protein sequence redundancy and similarity. In this study, an approach was developed for assessment of biological phenotype and metabolic status, as a functional complement to DNA sequence analysis. Fecal samples were prepared and analysed by tandem mass spectrometry and a homology-based meta-clustering strategy was used to combine peptides from multiple species into representative proteins. In total, 1

SUBMITTER: Cortes L 

PROVIDER: S-EPMC6471839 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature