Deep undepleted human serum proteome profiling toward biomarker discovery for Alzheimer's disease.

ABSTRACT:

Background

Blood-based protein measurement is a routine practice for detecting biomarkers in human disease. Comprehensive profiling of blood/plasma/serum proteome is a challenge due to an extremely large dynamic range, as exemplified by a small subset of highly abundant proteins. Antibody-based depletion of these abundant proteins alleviates the problem but introduces experimental variations. We aimed to establish a method for direct profiling of undepleted human serum and apply the method toward biomarker discovery for Alzheimer's disease (AD), as AD is the most common form of dementia without available blood-based biomarkers in clinic.

Methods

We present an ultra-deep analysis of undepleted human serum proteome by combining the latest 11-plex tandem-mass-tag (TMT) labeling

SUBMITTER: Dey KK 

PROVIDER: S-EPMC6472024 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature