NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.

ABSTRACT:

Purpose

This study aimed to investigate the effect of NPTX1 on the prognosis of gastric cancer (GC), as well as the metastatic process in GC.

Materials and methods

The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to analyze the association between NPTX1 expression and prognosis in GC. Quantitative real-time polymerase chain reaction and Western blots were applied to examine the expression of NPTX1 in GC cell lines and expression of genes in downstream pathways. The role of NPTX1 on the migration, invasion, adhesion, and proliferation of GC cell lines was investigated with the transwell assay, the adhesion assay, and the MTT assay. Immunofluorescence staining was used to observe the effect of NPTX1 knockdown on the morphology of cells.

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SUBMITTER: Yan H 

PROVIDER: S-EPMC6472287 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature