Targeted enrichment by solution-based hybrid capture to identify genetic sequence variants in barley.

ABSTRACT: In barley and other cereal crops, phenological diversity drives adaptation to different cultivation areas. Improvement of barley yield and quality traits requires adaptation to specific production areas with introgression of favorable alleles dependent upon precise identification of the underlying genes. Combining targeted sequence capture systems with next-generation sequencing provides an efficient approach to explore target genetic regions at high resolution, and allows rapid discovery of thousands of genetic polymorphisms. Here, we apply a versatile target-capture method to detect genome-wide polymorphisms in 174 flowering time-related genes, chosen based on prior knowledge from barley, rice, and Arabidopsis thaliana. Sequences were generated across a phenologically diverse panel of 89

SUBMITTER: Hill CB 

PROVIDER: S-EPMC6472358 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature