Tissue culture and next-generation sequencing: A combined approach for detecting yam (Dioscorea spp.) viruses.
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ABSTRACT:
SUBMITTER: Bomer M
PROVIDER: S-EPMC6472605 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Tissue culture and next-generation sequencing: A combined approach for detecting yam (<i>Dioscorea</i> spp.) viruses.
Bömer Moritz M Rathnayake Ajith I AI Visendi Paul P Sewe Steven O SO Sicat Juan Paolo A JPA Silva Gonçalo G Kumar P Lava PL Seal Susan E SE
Physiological and Molecular Plant Pathology. 20190101
<i>In vitro</i> culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates ...[more]
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