Dynamic filopodial forces induce accumulation, damage, and plastic remodeling of 3D extracellular matrices.

ABSTRACT: The mechanical properties of the extracellular matrix (ECM)-a complex, 3D, fibrillar scaffold of cells in physiological environments-modulate cell behavior and can drive tissue morphogenesis, regeneration, and disease progression. For simplicity, it is often convenient to assume these properties to be time-invariant. In living systems, however, cells dynamically remodel the ECM and create time-dependent local microenvironments. Here, we show how cell-generated contractile forces produce substantial irreversible changes to the density and architecture of physiologically relevant ECMs-collagen I and fibrin-in a matter of minutes. We measure the 3D deformation profiles of the ECM surrounding cancer and endothelial cells during stages when force generation is active or inactive. We further cor

SUBMITTER: Malandrino A 

PROVIDER: S-EPMC6472805 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature