Population-based analysis of cell-penetrating peptide uptake using a microfluidic droplet trapping array.

ABSTRACT: Cell-penetrating peptides (CPPs) have garnered significant attention as a method to introduce reporters and therapeutics into intact cells. While numerous studies have been performed identifying new CPP sequences, relatively little is known about their uptake efficiency at the single-cell level. Here, a droplet microfluidic trapping array was used to characterize CPP uptake across a population of single intact cells. The microfluidic device allowed for facile and rapid isolation and analysis of single-cell fluorescence in a 787-member overhead trapping array with > 99% droplet trapping efficiency. The permeability efficiencies of four different CPPs were studied and compared in HeLa cells. Population analysis was performed using linkage hierarchical cluster analysis by R programming to bin

SUBMITTER: Safa N 

PROVIDER: S-EPMC6472966 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature