Improved early development of porcine cloned embryos by treatment with quisinostat, a potent histone deacetylase inhibitor.

ABSTRACT: Recently, the modification of the epigenetic status of somatic cell nuclear transfer (SCNT) embryos by treatment with histone deacetylase inhibitors (HDACis) has made it possible to alter epigenetic traits and improve the developmental competence of these embryos. In the current study, we examined the effects of an HDACi, quisinostat (JNJ), on the in vitro development of porcine cloned embryos and their epigenetic nuclear reprogramming status. SCNT embryos were cultured under various conditions, and we found that treatment with 100 nM JNJ for 24 h post activation could improve blastocyst formation rates compared to the control (P < 0.05). Therefore, this was chosen as the optimal condition and used for further investigations. To explore the effects of JNJ on the nuclear reprogramming of ea

SUBMITTER: Taweechaipaisankul A 

PROVIDER: S-EPMC6473109 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature