Biochemical fingerprint of colorectal cancer cell lines using label-free live single-cell Raman spectroscopy.

ABSTRACT: Label-free live single-cell Raman spectroscopy was used to obtain a chemical fingerprint of colorectal cancer cells including the classification of the SW480 and SW620 cell line model system, derived from primary and secondary tumour cells from the same patient. High-quality Raman spectra were acquired from hundreds of live cells, showing high reproducibility between experiments. Principal component analysis with linear discriminant analysis yielded the best cell classification, with an accuracy of 98.7 ± 0.3% (standard error) when compared with discrimination trees or support vector machines. SW480 showed higher content of the disordered secondary protein structure Amide III band, whereas SW620 showed larger α-helix and β-sheet band content. The SW620 cell line also displayed higher nucle

SUBMITTER: Gala de Pablo J 

PROVIDER: S-EPMC6473482 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature