Detection of Circulating Tumor Cells Using Membrane-Based SERS Platform: A New Diagnostic Approach for 'Liquid Biopsy'.

ABSTRACT: The detection and monitoring of circulating tumor cells (CTCs) in blood is an important strategy for early cancer evidence, analysis, monitoring of therapeutic response, and optimization of cancer therapy treatments. In this work, tailor-made membranes (MBSP) for surface-enhanced Raman spectroscopy (SERS)-based analysis, which permitted the separation and enrichment of CTCs from blood samples, were developed. A thin layer of SERS-active metals deposited on polymer mat enhanced the Raman signals of CTCs and provided further insight into CTCs molecular and biochemical composition. The SERS spectra of all studied cells-prostate cancer (PC3), cervical carcinoma (HeLa), and leucocytes as an example of healthy (normal) cell-revealed significant differences in both the band positions and/or their

SUBMITTER: Kaminska A 

PROVIDER: S-EPMC6473992 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature