Differential proteomic comparison of breast cancer secretome using a quantitative paired analysis workflow.

ABSTRACT:

Background

Worldwide, breast cancer is the main cause of cancer mortality in women. Most cases originate in mammary ductal cells that produce the nipple aspirate fluid (NAF). In cancer patients, this secretome contains proteins associated with the tumor microenvironment. NAF studies are challenging because of inter-individual variability. We introduced a paired-proteomic shotgun strategy that relies on NAF analysis from both breasts of patients with unilateral breast cancer and extended PatternLab for Proteomics software to take advantage of this setup.

Methods

The software is based on a peptide-centric approach and uses the binomial distribution to attribute a probability for each peptide as being linked to the disease; these probabilities are propagated to a final protein

SUBMITTER: Brunoro GVF 

PROVIDER: S-EPMC6474050 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature