Single-cell correlations of mRNA and protein content in a human monocytic cell line after LPS stimulation.

ABSTRACT: The heterogeneity of mRNA and protein expression at the single-cell level can reveal fundamental information about cellular response to external stimuli, including the sensitivity, timing, and regulatory interactions of genes. Here we describe a fully automated system to digitally count the intron, mRNA, and protein content of up to five genes of interest simultaneously in single-cells. Full system automation of 3D microscope scans and custom image analysis routines allows hundreds of individual cells to be automatically segmented and the mRNA-protein content to be digitally counted. Single-molecule intron and mRNA content is measured by single-molecule fluorescence in-situ hybridization (smFISH), while protein content is quantified though the use of antibody probes. To mimic immune respon

SUBMITTER: Kalb DM 

PROVIDER: S-EPMC6474627 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature