Ontology highlight
ABSTRACT:
SUBMITTER: Jang Y
PROVIDER: S-EPMC6474810 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Jang Yunsu Y Son Heyjin H Lee Sang-Wook SW Hwang Wonseok W Jung Seung-Ryoung SR Byl Jo Ann W JAW Osheroff Neil N Lee Sanghwa S
Cell chemical biology 20190131 4
Topoisomerase II cleaves DNA at preferred sequences with different efficiencies; however, the mechanism of cleavage site selection is not known. Here we used single-molecule fluorescence assays that monitor several critical steps of DNA-topoisomerase II interactions, including binding/dissociation, bending/straightening, and cleavage/religation, and reveal that the cleavage site is selected mainly during the bending step. Furthermore, despite the sensitivity of the bending rate to the DNA sequen ...[more]