Hyperpolarized [6-¹³C,¹⁵N₃]-Arginine as a Probe for in Vivo Arginase Activity.

ABSTRACT: Alterations in arginase enzyme expression are linked with various diseases and have been shown to support disease progression, thus motivating the development of an imaging probe for this enzymatic target. ¹³C-enriched arginine can be used as a hyperpolarized (HP) magnetic resonance (MR) probe for arginase flux since the arginine carbon-6 resonance (157 ppm) is converted to urea (163 ppm) following arginase-catalyzed hydrolysis. However, scalar relaxation from adjacent ¹⁴N-nuclei shortens cabon-6 T ₁ and T ₂ times, yielding poor spectral properties. To address these limitations, we report the synthesis of [6-¹³C,¹⁵N₃]-arginine and demonstrate that ¹⁵N-enrichment increases carbon-6 relaxation times, ther

SUBMITTER: Cho A 

PROVIDER: S-EPMC6474818 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature