Ontology highlight
ABSTRACT:
SUBMITTER: Ahler E
PROVIDER: S-EPMC6474823 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Ahler Ethan E Register Ames C AC Chakraborty Sujata S Fang Linglan L Dieter Emily M EM Sitko Katherine A KA Vidadala Rama Subba Rao RSR Trevillian Bridget M BM Golkowski Martin M Gelman Hannah H Stephany Jason J JJ Rubin Alan F AF Merritt Ethan A EA Fowler Douglas M DM Maly Dustin J DJ
Molecular cell 20190404 2
Multiple layers of regulation modulate the activity and localization of protein kinases. However, many details of kinase regulation remain incompletely understood. Here, we apply saturation mutagenesis and a chemical genetic method for allosterically modulating kinase global conformation to Src kinase, providing insight into known regulatory mechanisms and revealing a previously undiscovered interaction between Src's SH4 and catalytic domains. Abrogation of this interaction increased phosphotran ...[more]